Upstream Processing
Cell Line Development
Cell line development is a critical process in biotechnology and pharmaceutical industries, involving the creation of stable clonal cell lines that produce high levels of desired proteins or antibodies.
At Biogentek, we specialize in providing state-of-the-art equipment that supports and enhances each phase of cell line development from single-cell cloning and high-throughput screening to facilitating the rapid identification and selection of high-producing cell lines.
Proof, not just Probability !!
Single Cell seeder, imager, clonality reports & cell viability with titre assessment
The STUDIUS ecosystem with the winning TRIO
VIPS PRO
*Single Cell Seeder
( Double lock assurance )
Clonality reports
Confluency data
Whole well imaging
21 CFR part 11 compliance module
Cell Metric X
High-throughput whole well imaging
Clonality reports
Confluency precision scanning
Automated evidence of clonality [*only machine in the world]
21 CFR part 11 compliance module
ICON
*Single instrument for all your CLD needs
Whole well imaging
Clonality reports
Confluency precision scanning
IgG titre assay
Viable cell density (VCD) analysis
Glycan assay
Aggregation assay [in the future]
21 CFR part 11 compliance module
Supplements for Cell line Development (CHO, HEK & IPSCs)
Single cells often require a support supplement to stay healthy & survive!
InstiGRO
*For CHO & HEK cells
InstiGRO– a unique formulation which supports the viability & growth of single cells, which normally are stressed out.
InstiSHAKE
InstiSHAKE- a supplement which supports your shake flask based scale up protocol.
InstiTHAW
InstiTHAW – supports the thawing of cells, after retrieval from the cell bank.
MatriClone
MatriClone– Laminin based matrix that supports the viability of single cell IPSC’s (Pluripotent stem cells)
Shakers & Incubators Shakers
Biogentek offers a comprehensive range of shakers, incubator shakers, rack shakers and pilot shakers from Kuhner AG Switzerland (pioneers in shaking technology since 1949).
Known for their precision, reliability and advanced technology, Kuhner Shakers are designed to meet the rigorous demands of modern laboratories and production facilities. Whether you are involved in research, development or large-scale production, our selection of Kuhner Shakers provides optimal solutions to enhance your workflows and achieve consistent, reproducible results.
The Magnetic Wonders From Switzerland !!
Machines that do not tire or retire !!
Incubator shaker ISF1-Z
Robust stackable Incubator Shakers with Conventional CFC Free Refrigeration for temperature control and now also with the latest heating and cooling concept using Sturdy and Lasting Independent Peltier based controls for Temperature & Humidity Control. Precise CO2 control & IQ/OQ. Stack 2 or 3 Machines or even more (if the height permits).
Incubator shaker LT-X
The Small Incubator Shaker with a big Heart (Space). Stackable, robust and space saving. Temperature control & shaking with or without precise CO2 control and humidity control.
Accepts flasks upto 6 liters.
Benchtop shaker Kuhner Kelvin⁺
A versatile piece of equipment which can be used as a stand alone incubator / a standalone shaker / even for cell culturing using Mass Flow Controllers and a Gas Distribution module.
Large capacity Incubation shaking ISF4-X
The real workhorse in high throughput laboratories. Optimize your work with several hundred small shake flasks or TPP / Falcon tubes or Scale up in large 5 Liter Flasks or culture in single use bags or even Media Thawing in controlled environment up-to 4 independent shaking machines housed in the large environmental cabinet. Ready with options of CO2 control & or humidity control, IQ/OQ & more .
Benchtop shaker LS-Z
Robust magnetically driven shaking machine which can be used in regular laboratory conditions or inside any other incubator or even in harsh conditions such as temperatures up to 100 degree Celsius and humidity up to 85 % Rh. A separate power unit & display enable the LSZ to be used in any incubator of your choice
Rack shaker SBM/SS-X
A brilliant solution for temperature controlled rooms for several small scale flasks simultaneously or large flasks (depending on heights). The SBM can accommodate any number of shakers from 1 up to 5 (max). Each shaker is independently controlled for rpm. Could be used as well for single use applications, for mixing in bags (using Special Trays), media mixing or thawing. (even in a cold room)
Pilot shaker RC-X
High capacity shaking machine for heavy loads, suitable for temperature controlled rooms or in ambient conditions . Shaking in large single used bags / containers for volumes up to 200 liters or higher. An excellent unit for mixing requirements or cell cultivation.
Single Use Bioreactors
Just shake & scale up
Kuhner is a leading innovator in the field of orbitally shaken bioreactor technology, used in upstream processing.
Their shaking bioreactors are engineered to deliver precise and consistent agitation, ensuring optimal mixing and aeration for cell culture . These systems are designed to enhance growth conditions, improve scalability and increase reproducibility in research and production environments.
Single Use Bioreactors- Shaken & Not Stirred !!
Easiest Scale up from 3 L to 2500 L in Single use !!
SB 10-X
W.V. = 4 L - 12 L
Benchtop orbital shaken bioreactor designed for the cultivation of human, mammalian and plant cells in a single-use bag. Supports a working volume range from 4 to 12 liters. Suitable for applications in research & process development. Also available with a 3L module for cultivation in the range of 1-3L.
SB 50-X
W.V. = 15 L - 50 L
It offers a working volume range of 15 to 50 liters, allowing for smooth scalability from microtiter plates to shaking flasks and further up to 50 liters.
It is versatile for use in research, process development and small scale production.
SB 200-X
W.V. = 50 L - 200 L
Offers a scalable working volume of 50 to 200 liters, supporting easy transition from SB10-X/SB50-X to SB2500-Z. The SB200-X can be utilized for a 50L bag as well with a slight modification. The bioreactor's consistent hydrodynamics ensure reproducible cultivation conditions, while integrated ports allow for feeding, inoculation, harvesting and sampling.
SB 2500-Z
W.V. = 500 L- 2500 L
Next-generation manufacturing-scale orbital shaken bioreactor (OSB).
With a working volume range of 500 to 2500 liters, this user-friendly system features quick setup times and is easily adaptable for production environments.
Fully compliant with GMP requirements and regulations.
Please do watch the shaken bioreactor in action !!
CO2 Incubators
Our CO2 incubators from LEEC are known for their advanced features, including a 200-degree Celsius sterilization cycle and innovative fan-less technology. The high-temperature sterilization cycle ensures complete eradication of contaminants, providing an ultra-clean environment crucial for sensitive cell cultures and research applications. The fan-less design further enhances this by minimizing contamination risks and ensuring uniform temperature distribution without disturbing the cell cultures. These cutting-edge features make LEEC CO2 incubators the best option on the market, offering unparalleled reliability and performance for your cell culture needs.
CO2 Incubators
P 190D
This 190-litre volume capacity is an advanced piece of laboratory equipment designed for precise control of temperature, humidity and carbon dioxide levels with 160 degree Celsius decontamination cycle, ideal for cell culture applications.
T 50S
This 50-litre compact volume CO2 incubator is a versatile and space-efficient solution for laboratories with limited space or for those needing to conduct smaller-scale experiments.
T 190S
This 190-litre large capacity CO2 incubator equipped with the unique 200 degree sterilization capability.
72 hours of graphical data logging and 100 text event logs, all conveniently saved as .csv files for easy viewing in MS Excel® from a removable SD card.
Fermenters & Bioreactors
Bionet is a prominent player in the upstream processing sector, specializing in the manufacture of high-quality autoclavable and stainless steel fermenters & TFF systems offering end to end solutions for your Bioproduction . Their fermenters are engineered to provide exceptional performance and durability.
Lab scale glass autoclavable fermenters
Bioreactors F0-Baby
An ideal entry level autoclavable benchtop bioreactor for a range of microbial and cell culture R&D activities for the agro-, food-, cosmetics- and biopharma industries. A versatile bioreactor for R & D with immense capabilities. Single controller can control up-to 3 bioreactors vessels.
Bioreactors F 1 Twin
The F1 series include autoclavable bench scale bioreactors/fermenters designed as per cGMP to meet the challenging and widely diverse R&D requirements and small-scale biomolecules production by using microbial and animal cells for biopharmaceutical-, food-, agricultural- and other biotechnological applications. Single controller & utility console for 2 bioreactors vessels.
Bioreactors F 1 Single
The F1 series include autoclavable bench scale bioreactors/fermenters designed to meet the challenging and widely diverse R&D requirements and small-scale biomolecules production by using microbial and animal cells for biopharmaceutical-, food-, agricultural- and other biotechnological applications. Designed to meet complete cGMP norms.
Industrial Bioreactors FS Industrial
FS are Bionet special Bioreactors, engineered and built to measure, when working volumes are bigger than 200 liters or more for specific needs which demand a special design, at any scale.
Bioreactors F 2
The models in the F2 series are the perfect solution for all those seeking for their first Steam In Place (SIP) stainless steel bioreactor/ fermenter for the scaling up of their bioprocesses and for small production applications. They are available in working volumes from 10 to 30 L. Possible to have inbuilt sterilization (no need for external steam/steam generator).
Bioreactors F 3
Bionet F3 models is a unique concept for those seeking for premium quality a Steam In Place (SIP) Bioreactor of industrial scale and conception, with the quality of a standardized product but with the flexibility to develop and scale-up new bioprocesses. Models for working volume range from 30 to 200 liters.
A next generation single use Fermenter series for Microbial Cultivation !!
Cell Tainer - Unique patented dynamics of movement-very high OTR/kLa values
CT-1
W.V. = Up to 3 L
CT-2
W.V. = 150 ml - 15 L
Features
- Save SIP and CIP validation steps by using single-use bags & also unique 2D movement that surpasses the OTR requirements/ kLa values of most fermenter systems in the world.
- Up to 400L h⁻¹ kLa owing to patented Unique 2D Rocking movement.
- Higher density and protein production levels are achieved at low shear (yeast/E. coli).
- Unparalleled working volume range, which reduces the number of seeding stages.
- The bag is free of moving parts, reducing costs and the risk of mechanical failure during operation.
- GMP compliance.
- Also available for cell culture applications!!
CT-3
W.V. = 30 L - 150 L
Please do watch the cell trainer in action !!
Carefully watch the unique 2D motion !!
Automated Cell Counters
Information will be updated shortly