Revolutionizing Bioprocessing with BIONET's F1 Bioreactor
A Leap Beyond Traditional Fed-Batch: Introducing Semi-Continuous Cultivation
Conventional batch and fed-batch processes are resource-intensive, often involving long turnaround times due to repeated cleaning, sterilization, and inoculum preparation. Bionet has engineered a smarter solution using its F1-3L bioreactor integrated with the Continuous Process Module (CPM) to enable semi-continuous bioproduction.
This innovative method was tested in a real-world setting using Pichia pastoris for recombinant protein expression and high cell density cultivation—simulating industrial production constraints.
Experimental Highlights: Efficiency Built into the Process
- Bioreactor Model Used: Bionet F1-3L Bioreactor with CPM
- Organism: Pichia pastoris – ideal for high-yield recombinant protein production
- Process Design:
- 6 cultivation cycles, each lasting 72–96 hours
- Two-thirds of the culture volume replaced in every cycle
- Low dilution rate (~0.014 h⁻¹) for extended productivity phase
- Smart control with online OD sensor and ROSITA® software
Why This Matters for Bioproduction
This semi-continuous approach offers significant advantages for labs and production environments:
- Minimized Downtime: Fewer cleaning and sterilization cycles
- Higher Productivity: Cells remain in productive phase longer
- Smart Monitoring: Real-time control and nutrient management via ROSITA®
- Scalability: Seamless transition from R&D to pilot and production scale
Conclusion: The Future of Efficient Bioprocessing
Bionet’s F1-3L bioreactor with CPM integration is not just a piece of equipment—it’s a leap forward in efficiency, flexibility, and process control. It empowers biotechnology and biopharma labs to produce more, waste less, and scale with confidence.
Ready to scale smarter? Contact Biogentek to explore how this system can revolutionize your downstream workflows.