Maximize Bioprocess Efficiency with Tezalon TFF (Single Use) Recirculation Testing
Insights from Biogentek
Biogentek is proud to highlight a breakthrough from our partner Tezalon in intelligent system optimization for Tangential Flow Filtration (TFF)—an innovation designed to maximize product yield, minimize loss, and streamline scale-up in your bioprocess operations.
The Value of Precise TFF Recirculation Volume
In downstream bioprocessing, every drop is precious. TFF, widely used for protein concentration and buffer exchange, depends critically on system design and operating parameters. One subtle—but powerful—factor is the recirculation volume: the amount of product circulating within the filter loop at any given time.
- Too much recirculation volume leads to unnecessary product hold-up and waste.
- Too little means unstable flow, risking inefficient filtration and membrane fouling.
- The right volume strikes the balance for robust, repeatable performance and optimal product recovery.
Tezalon’s Data-Driven Recirculation Testing
Tezalon's technical team performed focused studies to define the minimum vessel volume needed for stable crossflow—ensuring product integrity while safeguarding valuable biomolecules.
- Verified minimum recirculation vessel volume: 108 mL
- Systematic hold-up volume mapping of feed lines, retentate lines, and single-use filters
- Validation under real-world flow conditions to ensure no surprises in manufacturing runs
Tangible Benefits for Your Operations
- Maximize yield: More product reaches final formulation, not lost in dead zones.
- Minimize loss: Reduce hold-up and recirculation with precision engineering.
- Confident scaling: Validated parameters simplify transition from lab to production scale.
Why Tezalon’s Approach Stands Out
- Single-Use Innovation: Robust hygienic designs with minimal hold-up
- Real Data: Tested under realistic biomanufacturing conditions
- Scalability: Solutions validated from development through large-scale manufacturing
Ready for Results? Partner with Biogentek
Contact us today to see how the right TFF recirculation strategy, customized to your workflow, can deliver measurable savings and process improvements.
Frequently Asked Questions (FAQs)
What is Tangential Flow Filtration (TFF) used for in bioprocessing?
TFF is used for protein concentration and purification in downstream bioprocessing. It enables continuous filtration, reduces membrane clogging, and supports efficient buffer exchange.
Why is recirculation volume important in TFF systems?
Optimal recirculation volume helps balance flow stability and product recovery. Too much causes waste; too little disrupts performance and risks membrane fouling.
What makes Tezalon’s single-use TFF system unique?
Tezalon’s system is validated for hold-up volume, engineered for hygiene and efficiency, and tested under real-life manufacturing conditions for scalability.
How can Biogentek support my TFF optimization?
Biogentek offers expert technical support and customized solutions for Tezalon’s TFF systems—ensuring maximum yield and streamlined scale-up for your bioprocess.
Is Tezalon’s TFF system suitable for cGMP environments?
Yes. Tezalon’s TFF systems are compliant with cGMP manufacturing requirements, offering full traceability, single-use design, and scalable deployment.